Part of the project: The Ethiopian Wolf

Reproductive Knowledge of The Ethiopian Wolf Will Pave The Way to Reproductive Conservation: Freya’s Conclusions

Ethiopian wolves are medium-sized, territorial canids, endemic to the Ethiopian highlands. They live in family packs of up to 20 individuals, and within a pack there is a dominance hierarchy among both males and females. Within a pack, only the dominant pair breed. However, all pack members help rear the pups by guarding the den and regurgitating prey to the pups and subordinate females may allosuckle the pups. In addition, subordinate males may try to mate with dominant females in neighbouring packs.

Ethiopian wolves are classified as endangered and this species is under threat by habitat encroachment and disease, notably rabies and canine distemper, which are thought to be transmitted to wolves through domestic dogs that accompany local people living in Ethiopian wolf habitat. With less than 500 adults remaining, the Ethiopian wolf is Africa’s rarest canid. Several conservation measures have been suggested for this species, including reproductive management such as semen banking and captive breeding. However, knowledge about Ethiopian wolf reproductive physiology was previously lacking. My research therefore aimed to study the reproductive physiology of both male and female Ethiopian wolves through the collection of fresh faecal samples (in which hormones can be measured) and behavioural observations.

As there are no Ethiopian wolves in captivity, I had to conduct my research on a wild population. Bale Mountains National Park in Southern Ethiopia has the largest remaining population of Ethiopian wolves, and its population has been well studied for more than 20 years by the Ethiopian Wolf Conservation Programme (EWCP). For my research, I spent three 8 month field seasons (from July-March) in Bale, following wild wolves on foot and collecting faecal samples whenever a wolf defecated, as well as recording behavioural observations. The faecal samples were later sent to the Human Reproductive Sciences Unit in Edinburgh, where I analyzed them for oestradiol, progesterone, testosterone and cortisol.

I found that all dominant females showed oestrus behaviour including mating and/or an estradiol peak during the annual mating season, whereas none of the subordinate females did. This result suggests that subordinate Ethiopian wolves are hormonally reproductively suppressed. Both dominant and subordinate females had higher average progesterone levels during the dominant female’s pregnancy and two subordinate females allosuckled the dominant female’s pups, suggesting that subordinate females may become pseudopregnant. There were no significant differences between dominant and subordinate females’ cortisol levels, indicating that subordinates are not reproductively suppressed by increased stress hormones.

Over the course of the three field seasons, I observed dominant males preventing subordinates from mating with the dominant female several times, suggesting a behavioural mechanism of reproductive suppression. I did not find seasonal patterns in testosterone or cortisol levels, although dominant males had higher average testosterone and cortisol levels than did subordinates, which may be related to higher rates of aggression and mate guarding in dominant males of group-living canids, a pattern also reported in African wild dogs, Lycaon pictus.

I analyzed and wrote up these results as a thesis, which I submitted in March, and was examined on in June. I am also in the process of submitting these findings as papers to scientific journals. I hope the results found will contribute to the conservation of Ethiopian wolves and their future reproductive management. IBREAM, in collaboration with EWCP and the Ethiopian wildlife authorities, is working on an Ethiopian wolf semen banking project, which will hopefully help preserve genetic material from this rare charismatic canid.

Acknowledgements

I could not have conducted this research without financial support from IBREAM, the Prins Bernhard Cultuurfonds and Queen’s College Oxford. I would also like to thank the Ethiopian Wildlife Conservation Authority for their support of this research. Lastly I would like to thank my academic supervisors Prof. R. Millar, Prof. D. Macdonald, Dr. C. Sillero-Zubiri and Dr. M Paris for their support and supervision.

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Background and highlights

Team members

monique

Monique Paris

IBREAM Research Director

freya

Freya Van Kesteren

IBREAM PhD Student, University of Oxford

wenche.farstad

Wenche Farstad

IBREAM Advisory Board member, Norwegian University of Life Sciences (NMBU)

linda.penfold

Linda Penfold

IBREAM Advisory Board member, SEZARC

damien.paris

Damien Paris

James Cook University

Alemayehu Lemma

Alemayehu Lemma

Ethiopian based project partner Addis Ababa University

Gedlutessera

Gedlu Tessera

Field Assistant

muzeyenturkee

Muzeyen Turkee

Field Assistant

Antennahgirma

Antennah Girma

Field Assistant

TamamMohammed

Tamam Mohammed

Field Assistant

Collaborators: WildCRU, Addis Ababa University

Sponsors: IMV, WWF Netherlands